Spontaneous generation; He did this with two flasks filled with broth. One was sealed and the other was unsealed. The unsealed one started to grow micro organism.
They were scientists that simulated conditions that were present on early earth. The conducted experiment was. Water and gases were sealed inside a beaker.
According to Mulkidjanian, the debate about whether life could arise from chemical reactions began to change when scientists started to question the atmospheric conditions used by Miller and Urey. In their famous experiment, Miller and Urey replicated the early Earth atmosphere with a mixture of methane, hydrogen, ammonia and water vapor. This mixture, along with some “sparks” which simulated lightning, led to the formation of amino acids. With this setup, Miller and Urey assumed that the early Earth had a reducing atmosphere, which meant it had large amounts of hydrogen and almost no oxygen.
While Oparin himself was unable to conduct experiments to test any of these ideas, later researchers tried. In 1953, Stanley Miller attempted an experiment to investigate whether chemical self-organization could have been possible on pre-historic Earth. The Miller–Urey experiment introduced heat (to provide reflux) and electrical energy (sparks, to simulate lightning) into a mixture of several simple components that would be present in a reducing atmosphere. Within a fairly short period of time a variety of familiar organic compounds, such as amino acids, were synthesised. The compounds that formed were somewhat more complex than the molecules present at the beginning of the experiment.
There is no fundamental difference between a living organism and lifeless matter. The complex combination of manifestations and properties characteristic of life must have arisen as a part of the process of the evolution of matter. Taking into account the recent discovery of methane in the atmospheres of Jupiter and the other giant planets, Oparin suggested that the infant Earth had possessed a strongly reducing atmosphere, containing methane, ammonia, hydrogen and water vapor. In his opinion, these were the raw materials for the evolution of life.
THROUGH CHANGES IN THE EARTHS ATMOSPHERE THE EARTH COOLED CONDITIONS WERE FAVORABLE FOR MICRO ORGANISMS. LIFE BEGAN TO GROW AND OTHER ORGANISMS BEGAN TO EVOLVE.