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Case Study #4

Published on Nov 21, 2015

Streptococcus pyogenes case study for mlt course 68k, class 040

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PRESENTATION OUTLINE

1.

Case Study #4

Sore Throat
Photo by SnaPsi Сталкер
2.

Patient Sample

  • Throat Swab
Photo by goldtoe
3.

Media

  • SBA
Photo by foxypar4
4.

Why?

  • Supportive
  • Differential
Photo by Sebastiaan ter Burg
5.

Setup

  • Stab agar 4x
  • 4 Quadrant 
Photo by One lucky guy
6.

Incubation

Photo by Putneypics
7.

Results

Beta Hemolysis, White, Small
8.

Gram Stain

GPC, P/C
Photo by Marc Perkins - OCC Biology Department
9.

Let's Do Some Tests

  • Catalase
  • CAMP
  • Esculin/PYR
Photo by ~Brenda-Starr~
10.

Catalase

  • Strep vs. Staph
  • Result: No Bubbles, Negative
  • = Streptococcus 
11.

CAMP

  • Differentiate Beta Strep
  • Result: Negative
  • = Further Testing
Photo by Al_HikesAZ
12.

Esculin/PYR

  • Enterococcus Spp vs. Strep
  • Esc: Negative
  • PYR: Positive
13.

Streptococcus pyogenes

the results are in
14.

Penicillin

There when you need them
Photo by DVIDSHUB
15.

Allergies?

  • Azithromycin
  • Clarithromycin
  • Erythromycin
Photo by Nomadic Lass
16.

Susceptibility Testing

  • Required if not using Penicillin
  • Disk Diffusion
  • Broth Dilution
  • Agar Dilution
Photo by mostly*harmless
17.

Questions??

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