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Gel Electrophoresis
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Presentation and example of gel ectrophoresis
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PRESENTATION OUTLINE
1.
WHAT IS GEL ELECTROPHORESIS?
Photo by
CodonAUG
2.
IT IS A TECHNIQUE THAT ALLOWS SCIETISTS TO SEPERATE DNA ACCORDING TO ITS SIZE.
3.
WHAT IS IT USED FOR?
4.
Gel electrophoresis is used for forensic analysis, parentity testing, diagnosng genetic diseases, and animal breeding.
5.
WHAT DO I NEED TO SEPERATE DNA MOLECULES?
DNA samples
Agarose
Buffer
Apparatus machine
Power supply
Micropipet
Stain to see DNA
6.
STEPS TO CARRY OUT THE EXPERIMENT
Dilute buffer
Mix agarose with buffer to make gel
Heat in microwave
Cool
Pour solution into the trays
In 20 minutes remove the caps and comb
Load DNA samples into wells
Place cover
Connect power source and perform electrophoresis
Visualize results.
7.
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12.
EXPERIMENT RESULTS
WHODUNNIT?
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andrewrennie
13.
Lane 1 Crime Scene DNA 1
Lane 2 Crime Scene DNA 2
Lane 3 Suspect 1-1
Lane 4 Suspect 1-2
Lane 5 Suspect 2-1
Lane 6 Suspect 2-2
14.
SUSPECT 2?!
Olivia Hunt
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