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Gel Electrophoresis

Presentation and example of gel ectrophoresis

PRESENTATION OUTLINE

WHAT IS GEL ELECTROPHORESIS?

Photo by CodonAUG

IT IS A TECHNIQUE THAT ALLOWS SCIETISTS TO SEPERATE DNA ACCORDING TO ITS SIZE.

WHAT IS IT USED FOR?

Gel electrophoresis is used for forensic analysis, parentity testing, diagnosng genetic diseases, and animal breeding.

WHAT DO I NEED TO SEPERATE DNA MOLECULES?

  • DNA samples
  • Agarose
  • Buffer
  • Apparatus machine
  • Power supply
  • Micropipet
  • Stain to see DNA

STEPS TO CARRY OUT THE EXPERIMENT

  • Dilute buffer
  • Mix agarose with buffer to make gel
  • Heat in microwave
  • Cool
  • Pour solution into the trays
  • In 20 minutes remove the caps and comb
  • Load DNA samples into wells
  • Place cover
  • Connect power source and perform electrophoresis
  • Visualize results.

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EXPERIMENT RESULTS

WHODUNNIT?
Photo by andrewrennie

Lane 1 Crime Scene DNA 1
Lane 2 Crime Scene DNA 2
Lane 3 Suspect 1-1
Lane 4 Suspect 1-2
Lane 5 Suspect 2-1
Lane 6 Suspect 2-2

SUSPECT 2?!