The route by which this organism is spread is from human to human in the form of aerosol droplets. When inside the host the organism’s primary site of pneumococcal colonization is the nasopharynx. From this site it can aspire to the lungs, eventually spread to the blood and traverse the blood-brain barrier to the meninges, once inside the blood it can cause infections throughout the body.
A gram stain is performed from the sputum of the infected patient
The presence of neutrophils and greater than ten gram-positive diplococci
When on blood agar the organism should exhibit alpha-hemolysis, which is characterized as a zone of green coloring around the colonies of bacteria on the agar
The streaked organisms must also exhibit bile or optochin sensitivity