THE D.O. EFFECT

QUESTION:

INTRODUCTION

• DO measures the amount of gaseous oxygen in an aquatic solution
• DO shows us if aquatic organisms can prosper or not
• We measured the DO level and air temperature at 3 spots in Royal Creek
• Total dissolved gas concentrations in water can't exceed 110%
• Oxygen is the most well-established water quality indicator

METHODS

• For 10 weeks we went down to the Royal Creek once a week
• We measured the DO level and air temperature at three spots
• We filled our glass tubes with the water and put the DO capsules in them
• We shook up the tube for five minutes
• Then we looked at a chart to find the DO of the water.

START OF EXPERIMENT

• At this time it was much warmer outside.
• When we reached the creek we collected data with ease.
• The creek was low so we could place our books and equipment
• on the rocks while taking temperatures and collecting water.

WEEK 1 9/5/2013

WEEK 2 9/12/2013

WEEK 3 9/19/2013

WEEK 4 9/26/2013

WEEK 5 10/1/2013

WEEK 6 10/10/2013

The Change

When we got back to school after fall break we noticed that the water levels of the creek had risen tremendously and it had its effects of the DO levels we calculated.

WEEK 9 11/1/2013

WEEK 7 10/21/2013

WEEK 8 10/25/2013

WEEK 10 11/7/2013

The DO Capsules:

The method for the determination of DO is based upon the oxidation of an idol in an acidic citrate buffer by DO in the water being tested. Amidol is 2, 4-diamino phenol dihydrochloride with the chemical formula C6H8N2O-2HCI
Citrate
Buffer total alkalinity method
Amidol + O2 + 2H = oxidized Amidol + 2H2O

Spot 1

Spot 2

Spot 3

CONCLUSION

• While it was warm out side and the water level was less,
• the DO level was less.
• While it was cooler outside after fall break, the water
• level had risen and so had the DO levels.